Objective : The purpose of this study was to evaluate the effect for running shoes with resilience of midsole on biomechanical properties· Methods : 10 healthy males who had no history of injury in the lower extremity with an average age of 26·5 year(SD=1·84), height of 172·22cm(SD=4·44) and weight of 67·51 kg(SD=6·17) participated in this study· All subjects ran on the treadmill wearing three different running shoes· Footpressure data was collected using Pedar-X system(Novel Gmbh, Germany) operating at 100 Hz· Surface EMG signals for biceps femoris, rectus femoris,vastus lateralis, medial lateralis, tibialis anterior, medial gastrocnemius, soleus and peroneus longus were acquired at 1000 Hz using Bignoli 8System(Delsys Inc·, USA)· To normalize the difference of the magnitude of muscle contractions, it was expressed as a percentage relative to themaximum voluntary contraction (MVC)· The impact resilience of the midsole data was collected using Fastcam SA5 system(Photron Inc·, USA)· Collected data was analyzed using One-way ANOVA in order to investigate the effects of each running shoes· Results : TPU midsole was significantly wider in contact area than EVA, TPE midsole in midfoot and higher in EMG activity than EVA midsole atbiceps femoris· TPE midsole was significantly wider in contact area than EVA midsole in rearfoot and higher in peak pressure than EVA midsole inforefoot· EVA midsole was significantly higher in EMG activity than TPU midsole at tibia anterior· In medial resilience of midsoles, TPE midsole wassignificantly higher than EVA, TPU midsole· Conclusion : TPU midsole can reduce the load on the midfoot effectively and activate tibialis anterior, biceps femoris to give help to running·