Human pluripotent stem cells(hPSCs) are useful resource for cell replacement therapy and modeling of human diseases. Recently, genome editing technologies have has been applied in hPSCs that are rapidly developed for targeted gene function of the human genome. These technologies including zinc finger nucleases(ZFNs), transcription activator like effector nucleases (TALENs) and clustered regulary interspaced short palindromic repeat(CRISPR)/CRISPR associated protein 9(CAS9) RNA guided nucleases are genetically modified tools which have potentials for diverse biolohical research fields. In this review, we discussed an overview of genome editing merhods and understanding their characters and drawback of this manipulation.