Background: Human placental extract (HPE) is a substantially reservoir of bioactive molecules and various growth factors. It has been approved and is now being used widely for improvement of fatigue. However, the mechanisms responsible for HPE-induced hair growth mechanisms are not fully understood yet. In an effort to develop new therapies to enhance hair growth, we screened human placental extract. Objectives: In the present study, we observed that HPE exerted hair growth-promoting activities and induced β -catenin via inhibition of GSK-3β by phosphorylation in human dermal papilla cells Methods: HPE significantly increased the viability of hDPCs in a concentration-dependent manner, as observed by bromodeoxyuridine (BrdU) assay. HPE also significantly increased alkaline phosp--hatase (AP) expression levels. Increased β-catenin levels and inhibition of GSK-3β (Ser9) by phosphorylation suggest that HPE ind--uces hair growth in hDPCs. Also, we compared the effects of HPE treatment alone with HPE treatment supplemented with minoxidil (MXD) Results: We found that HPE plus MXD synergistically inhibited GSK-3β by phosphorylation (Ser9) and regulated IGFR1 and AKT, which are upstream proteins of GSK3β in hDPCs. In particular, we revealed that HPE is effective in inducing hair elongation in rat vibrissae hair follicles, and that HPE leads to a delay in catagen progression Conclusion: Overall, our findings suggest that HPE promotes hair growth and could offer a new option for clinical treatment