Background: Platelet rich plasma(PRP) is widely used for hair growth treatment. However, the mechanisms of PRP and combination of growth factors are not fully understood yet. In an effort to develop new therapies to enhance hair growth, we screened PRP and combination of growth factors of it Objectives: We investigate the growth factors in PRP and compare the effect on hair growth between PRP and combination of growth factors Methods: We examined the effect of PRP and combination growth factors which mixed in different ratio on human dermal papilla cells and C57BL6/J mice using western blot analysis, real-time PCR and immunocytochemistry. Also, we did organ cultures of combining different growth factors for measuring hair length. Results: Combination of EGF and PDGF-AA (1:1 ratio) induced synergistic hair growth and anagen induction activity by regulation of hair growth-related signaling pathways and molecules in hDPCs and C57BL6/J mice model. Conclusion: Combination of EGF and PDGF-AA (1:1 ratio) can be further pharmaceutically developed as a therapeutic material against hair loss and alopecia in human.