Background: Human placenta extract (HPE) has been used for alleviation of fatigue, anti-melanocyte activity, and antiaging effects. However, it has not yet been studied in hair growth. Objectives: We evaluated the effect of HPE on hair growth in vitro by observing its effect on human dermal papilla cells (DPCs). The purpose of this research was to define HPE promotion of induction of anagen hair growth in telogen using C57BL/6J mice and to understand the molecular mechanisms of HPE and minoxidil (MXD) action on hair growth. Methods: We examined the effect of HPE and MXD on C57BL6/J mice using hematoxylin and eosin (H&E) staining, quantitative histomorphometry, hair growth scoring, immunohistochemistry, and immunofluorescence with the dorsal skins of C57BL/6J mice. Results: We found that HPE synergistically augmented the effects of MXD, a promoter of hair growth. In particular, the histomorphometric analysis data indicated that subcutaneous injection of HPE induced an earlier anagen phase and prolonged the anagen phase, and also stimulated increases inboth the number and size of hair follicles in the groups treated with HPE and HPE plus MXD. Conclusion: From this study, we conclude that HPE may increase β-catenin and Wnt3a expression levels. Overall, our findings suggest that HPE in combination with MXD may have hair growth-promoting activity and it could serve as a novel therapeutic treatment of alopecia or baldness in humans.