Objectives : The purpose of this study was to investigate the relationship between psychosocial stress and depression symptoms in dental hygiene freshman. Methods : A self-reported questionnaire of 45 questions was filled out by 207 dental hygiene students in Gyeongnam from December 9 to 11, 2013. Data were analyzed using SPSS WIN 18.0 program for descriptive statistics, t-test, ANOVA. Results : Religion tended to influence on positive effects on academic stress because those having religion had less academic stress. Economic condition also influenced on physical and mental stress in the students(8.52±5.63)(p=0.000). Low economic status influenced on academic problems(p=0.022), family problems(p=0.014), rational relationship problems(p=0.002), and interpersonal problems(p=0.007). There was no significant difference between future problems(p=0.125) and self-efficacy(p=0.076). The physical symptoms included digestive disorders(16.73±5.37), cardiac symptoms(14.61±5.51), headache(9.46±3.59), insomnia(8.32±3.67) (p=0.000). The mental symptoms included negative emotions(15.07±3.94)(p=0.000). Conclusions : In order to reduce the psychosocial stress symptoms in dental hygiene freshman, it is important to develop the stress debriefing program in the future.