For this study, we collected fishes of seven times at the 51 sites by stream orders (2-6) from November 2007 to September 2010. In the results, 55 species belonging 12 families were collected. And there were one species (1.8%) of Natural monument, 6 species (10.9%) of endangered and 22 species (40.0%) of Korea endemic. Dominant species was Zacco koreanus and subdominant species was Zacco platypus in whole. Stream orders (2-6) increased with increasing species, diversity (H′), evenness (EI), richness (RI), tolerant (TS), omnivore (OS) and decreasing individual, dominance (DI), sensitive (SS) insectivore (IS), total IBI score. Principal component analysis results showed location of variable (species, individual, community index, relative abundance of tolerant, sensitive, omnivore, insectivore species) and each site changes in upstream and down-stream changes in their distribution. Correlation analysis showed significant differences between stream orders (2-6) and variable (species: Sp, diversity index: H′, omnivore species: OS, stream depth: SD, stream width: SW and water temperature: WT).