Single port laparoscopic surgery has been utilized in various gynecologic fields according to the development of laparoscopic equipment and techniques and the increasing demand for minimally invasive surgery. Although several studies of SP-LS for ectopic pregnancy have been reported, most of these studies were performed only in hemodynamically stable women with tubal pregnancy and excluded other types of ectopic pregnancy. The clinical usefulness of these prior studies is limited because of the difficulty in differentiating the type of ectopic pregnancy and the considerable rate of hemodynamic instability observed in this population. To obtain more clinically relevant data, we evaluated the feasibility and safety of SP-LS for ectopic pregnancy regardless of the hemodynamic stability and the type of ectopic pregnancy A prospective case-control study of total 106 women who underwent SP-LS or conventional LS for a suspected ectopic pregnancy was performed at a University teaching hospital from January 2009 to March 2012. 26 women underwent SP-LS and 80 women underwent conventional LS. There were no statistical differences between the groups in terms of demographic characteristics, operating time, postoperative hemoglobin change, return of bowel activity, hospital stay, or complication rate. There were no cases of additional trocar use or conversion to laparotomy. Of five women with heterotopic pregnancy, one underwent SP-LS, and three underwent conventional LS for tubal pregnancy, which all resulted in vaginal delivery without obstetrical complication; one woman received SP-LS for cornual pregnancy and had an ongoing pregnancy. SP-LS for ectopic pregnancy is feasible and safe in women with regardless of the type of ectopic pregnancy and hemodynamic stability. However, further work is needed to confirm this conclusion and to demonstrate any advantage of SP-LS for ectopic pregnancy.