Epidemiological studies have focused on the deleterious effects of smoking on human health. Of particular the number of epidemiological studies reported that cigarette smoke decreases bone mineral density (BMD) and increases the risk of bone fracture and is a risk factor for osteoporosis. Maternal smoking during pregnancy results in a variety of adverse developmental outcomes with intrauterine growth restriction. Moreover, maternal smoking is associated with future onset of attention deficit hyperactivity disorder, cardiovascular diseases and obesity in offspring. However, little is known about the effect of maternal smokE during pregnancy on bone mineral density in offspring. Pregnant CD-1 mice were exposed to cigarette smoke (1 or 2 cigarettes/day, 5 days/week) (smoke group) or sham exposed (control group) throughout the pregnancy. After delivery, nursing dams and offspring were kept together in individual cages for 21 days, which corresponds to the lactation period. At weaning, the fourth lumbar vertebral body from each of offspring was scanned with micro-CT apparatus. Trabecular parameters including bone volume fraction (BV/TV, %), thickness (mm), separation (mm), and number (1/mm) were evaluated. The BMD was also measured. There were no differences in trabecular bone volume fraction, thickness, separation and number and the BMD between offspring from control and 1 cigarette smoke dams. However, trabecular bone volume fraction, thickness, and number, and the BMD were significantly lower, but trabecular separation was higher in offspring from 2 cigarettes smoke dams compared with offspring from control dams. In this study, maternal smoking during pregnancy decreased the bone mineral density and disturbed the microarchitecture of bone in offspring. Further studies are needed to evaluate the long term effect of maternal smoke during pregnancy on bone of offspring.