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KCI 등재
중저분자 키토산이 인간암세포 저온저장에 미치는 효과
Effect of Medium Molecular Weight Chitosan on Cold Preservation of Human Cancer Cell Lines
김정균 ( Jeong Gyun Kim ) , 오규석 ( Gyu Suk O ) , 고용훈 ( Yong Hun Go ) , 장솔지 ( Sol Ji Jang ) , 박제권 ( Jae Kweon Park ) , 김대영 ( Daeyoung Kim ) , 신수영 ( Su Young Shin ) , 황유진 ( You Jin Hwang )
UCI I410-ECN-0102-2014-500-002003841

Although cryopreservation has been used to keep viability of cells, it has a severe problem with damaging cells while freezing and thawing them. So we have tried long-term reservation of cells to solve the problem. In this study, we stored three kinds of cells (Hep3B, A549, 293T) in the presence of three different medium molecular weight chitosans (MMWCs) in concentration of 0.05%, 0.01%, 0.005% and 0.001%, respectively, under reservation for a certain period of time. We named them P (MW: 7.6 kDa), B (MW: 5.6 kDa), S (MW: 3.5 kDa) respectively. Then we measured viability of cells with FACS and ELISA after 2, 3 and 4 days respectively. And we measured proliferation of cells with cell proliferation ELISA, BrdU (colorimetric). Our data suggest that viability of cells increase in concentration dependent manner of MMWCs. Subsequently this study demonstrated that MMWCs (MW: 3~7 kDa)are significant factors acting in long-term reservation of cells. However MMWCs have no clear effect on proliferation of cells. Further studies are needed to perform in long-term storage of cells using MMWCs including other polysaccharides to enhance an efficiency of storage.

[자료제공 : 네이버학술정보]
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