Objectives: Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP (Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods: Nitric oxide (NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-α were measured by western blotting assay. Results: CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-α in C6 glioma cells. Conclusions: These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-α. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.