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대계 약침액의 C6 신경교종 세포에 대한 이주 억제 효과
The Anti-Migratory Effect of Cirsium japonicum Pharmacopuncture in C6 Glioma Cell
박주연 ( Juyeon Park ) , 이강파 ( Kangpa Lee ) , 장해룡 ( Haeryong Chang ) , 문진영 ( Jinyoung Moon )
UCI I410-ECN-0102-2014-500-001951719

Objectives: Cirsium japonicum is a traditional Korean medicine that has been used in the treatment of inflammatory diseases such as appendicitis, hepatitis, pulmonary abscess and tumor. The aim of study was to elucidate anti-migratory activity of CJP (Cirsium japonicum pharmacopuncture) through regulation of inflammatory mediators in C6 glioma cell. Methods: Nitric oxide (NO) production was determined by using nitrite assay. The cell migration was analyzed by wound-healing assay and Boyden chamber assay. The expression levels of iNOS, and protein kinase C(PKC)-α were measured by western blotting assay. Results: CJP showed a significant decrease on NO production. Moreover, glioma cell migration was effectively suppressed by CJP. Furthermore, CJP inhibited the expressions of iNOS and PKC-α in C6 glioma cells. Conclusions: These results suggest that CJP inhibits glioma cell migration and iNOS expression through regulation of PKC-α. Therefore, it is expected that CJP could be an effective agents for blocking malignant progression of glioma.

[자료제공 : 네이버학술정보]
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