This study examines the rustling sound characteristics of electrospun nanofiber web laminates according to layer structures. This study assesses mechanical properties and frictional sounds (such as SPL); in addition, Zwicker`s psychoacoustic parameters (such as Loudness (Z), Sharpness (Z), Roughness (Z), and Fluctuation strength (Z)) were calculated using the Sound Quality Program (ver.3.2, B&K, Denmark). The result determined how to control these characteristics and minimize rustling sounds. A total of 3 specimens` frictional sound (generated at 0.63 m/s) was recorded using a Simulator for Frictional Sound of Fabrics (Korea Patent No. 10-2008-0105524) and SPLs were analyzed with a Fast Fourier Transformation (FFT). The mechanical properties of fabrics were measured with a KES-FB system. The SPL value of the sound spectrum showed 6.84~58.47dB at 0~17,500Hz. The SPL value was 61.2dB for the 2-layer PU nanofiber web laminates layered on densely woven PET(C1) and was the highest at 65.1dB for the 3-layer PU nanofiber web laminates (C3). Based on SPSS 18.0, it was shown that there is a correlation between mechanical properties and psychoacoustic characteristics. Tensile properties (LT), weight (T), and bending properties (2HB) showed a high correlation with psychoacoustic characteristics. Tensile linearity (LT) with Loudness (Z) showed a negative correlation coefficient; however, weight (T) with Sharpness (Z) and Roughness (Z), and bending hysteresis (2HB) with Roughness (Z) indicated positive correlation coefficients, respectively.