Background: Alopecia is a common disorder with significant negative impact on their social and psychological well-being. There are many modalities to treat various alopecia, such as oral finasteride, topical minoxidil, triamcinolone intralesional injection, excimer laser and so on. However, the treatment result of these treatment are not always satisfactory. Objectives: The purpose of this study is to evaluate efficacy and safety of sequential treatment, Platelet-rich plasma injection, follwed by mesotherapy in various alopecia. Methods: Included in this study were males and females between the ages of 20 and 60 years, in good health. Two independent investigators evaluated clinical improvement using a quartile grading scale by comparing digital photographs taken prior to treatment and at 3 months after the last treatment. Results: The result is a relatively clinical improvement in hair count and hair thickness was demonstrated in all the patients. There were no significant side effects except mild pain, transient bleeding at injection or microneedling site. In vitro study, PRP showed anti-apoptotic effects through upregulation of BCl-2 expressions in cultured dermal papilla cells. Conclusion: Taken together, the treatment of various alopecia using sequential treatment, platelet rich plasma injection, followed by mesotherapy promote hair growth.