Background: Hair loss has a great impact on a person`s self-respect, mental health, and quality of life. Thus, it is important to develop new therapeutic materials that prevent hair loss and enhance hair growth. Objectives: This study was aimed to investigate the effects of recombinant growth factors mixture (RGFM) on hair growth promotion in vitro and in vivo. Methods: We evaluated cell proliferation in cultured human dermal papillary cells (DPCs) by MTT assay and determined cell migration in DPCs using Phage contrast microscopy and measured the expression levels of extracellular signal-regulated kinase (ERK), Akt, GSK-3β, and β-catenin by Western blot analysis. We also performed topical application onto the back skins of the 7 week-old C57BL/6 mice after depilation. Results: RGFM promotes the proliferation and migration and survival of human DPCs by activating both ERK and Akt at 1,000 ng/ml concentration. Moreover, RGFM induced the proliferation of human DPCs by the upregulation of β -catenin accompanied by inhibition of GSK-3β. In addition, RGFM treatment on the back skins of C57BL/6 mice significantly promoted hair growth compared with control treatment. Conclusion: Thus, we suggest that RGFM promotes human hair growth through these proliferative effects on human DPCs.