Background: Diabetes is characterized by chronic hyperglycemia, and hyperglycemia could Increase Reactive Oxygen Species (ROS) production from the mitochondrial electron transport chain. The formation of ROS in cells induces oxidative stress and activates oxidative damage-inducing genes. There is no research on oxidative damage-related genes AKR1C3 protein levels in human diabetic skin. We explored the expression of AKR1C3 in diabetic skin compared with normal skin tissue. Objectives: To compare the expression of AKR1C3 in normal skin versus diabetic skin. Methods: AKR1C3 expression was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was carried out to analyze AKR1C3 expression in the 6 diabetic skin tissue samples. Results: The western blotting showed a significant reduction in AKR1C3 protein expression in diabetic skin tissue compared to normal tissue. Immunohistochemical examination of AKR1C3 showed that it was weakly expressed in all diabetic skin samples. Conclusion: We believe that AKR1C3 is related to diabetic skin in an altered metabolic state which elevates ROS production.