Purpose: The purpose of this study was to identify the relationships between the level of awareness of dental hygiene and practice of dental hygiene in pregnant women. Methods: This study was conducted through a survey on 170 pregnant women who visited obstetric clinics in five the general hospitals in B city and K city. The data were collected by self-reporting questionnaires and analysed by the SPSS WIN 17.0 program using descriptive statistics, t-test, ANOVA, Pearson correlation coefficient, and stepwise multiple regression. Results: The mean score of awareness of dental hygiene was 97.4. The mean score of practice of dental hygiene was 122.8. Education levels have a significant effect on practice of dantal hygiene. A significant positive correlation between awareness of dental hygiene and practice of dental hygiene was found. Conclusion: It is suggestive that effective educational programs are in need to increase the level of practice of dental hygiene and level of awareness of dental hygiene.