Objectives: The purpose of this study was to examine the satisfaction level of dental hygiene students with their major and clinical practice in an effort to have a good understanding of dental hygiene students. The subjects in this study were 226 juniors who were selected from among dental hygiene students at four different colleges located in Busan and South Gyeongnam Province. Methods: The collected data were analyzed by the SPSS WIN 15.0. Statistical data on frequency and percentage were obtained to figure out the general characteristics of the students. To grasp their satisfaction level with clinical practice and major, another statistical data on mean and standard deviation were obtained, and t-test and one-way ANOVA were carried out and correlation analysis and multiple regression analysis. Results: As for the relationship between general characteristics and satisfaction with clinical practice, their collective average was 3.29. The dental hygiene students who were more satisfied with their major expressed higher satisfaction with the practicums, the content of practice and clinical practice. In relation to the impact of the satisfaction of the dental hygiene students with major on their satisfaction with clinical practice, the students who were more satisfied with social awareness and with the courses expressed better satisfaction with clinical practice. Conclusions: Given the findings of the study, the departments of dental hygiene that nurture professionals should carefully consider how to raise the satisfaction of students with the courses that affect the improvement of satisfaction with major and clinical practice.