The aim of the present work is to prepare and evaluate the oral mucoadhesive sustained release nanoparticles of lactocin from Lactobacillus plantarum(CBT-LP2) by simplifying its administration, improving its function and dose related safety for smart probiotic nanoparticles as a nanofood. The influences of the spray drying parameters and the type of nanoparticles on the characteristics of nanoparticle coated probiotic nanofood(NCPN) were investigated by using a factorial design 32. In vitro lactocin release demonstrated the influence of the nanoparticle coating on the dissolution profiles of CBT-LP2. NCPN 5 presented a protective effect on the gastrointestinal mucosa.