식품의 안전성 확보와 저장성 증진을 위해 천연물 소재 식품 보존제를 개발하기 위하여 광곽향, 구절초, 금전초, 비수리, 속단, 황벽나무피, 향유, 계피의 병원성균(L. monocytogenes, monocytogenes, S. aureus, B. cereus, L. plantarum, A. hydrophila subsp. hydrophila, P. fluorecens, V. parahaemolyticus, E. coli, S. enterica subsp. enterica, S. aureus)항균활성을 검토하였다. 계피 추출물은 모든 공시 균주에 대해 금전초는 E. coli O157: H7를 제외한 공시균주에 대해 강한 항균활성을 나타내었다. 금전초, 비수리, 계피 추출물의 DPPH 라디칼 소거능은 각각 36.35, 95.85, 77.30%를 나타내었다. 천연물 혼합용액 (0.1% chitosan + 100 ppm 자몽종자추출물 + 0.2% adipicacid)에 각각 금전초와 계피 추출물을 농도별(0, 0.1, 0.2, 0.3, 0.4, 0.5%)로 첨가하여 살균소독 표준균주에 대한 항균효과를 측정한 결과 S. aureus는 약 5~6 log cycle 감소한 반면, E. coli는 검출되지 않았다. 천연물 혼합용액에 각각 금전초(P1) 또는 계피 추출물(P2) 0.1% 첨가하여 식중독 및 식품부패 세균(gram(+): L. monocytogenes, S. aureus, B.cereus 그리고 gram(-): Sal. enterica subsp. enterica, V. parahaemolyticus, A. fluorescens, E. coli)을 5분간 처리한 결과 생균수는 검출되지 않았다.

The antimicrobial activities of 8 extracts from medicinal plants (Pogostermon cablin, Bentham, Chrysanthemum zawadskii, Lysimachia christinae Hance, Lespdeza cuneata G. Don, Phlomis umbrosa Turcz, Phellodendron amurense Ruprecht, Elshotzia ciliate Hylander and Cinnamomum zeylanicum) were investigated by paper disc method against various pathogens (Listeria. monocytogenes, Stapylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica, Hydrophila hydrophila, Pseudomonas. fluorecens and Vibrio parahaemolyticus), Their DPPH radical scarvenging activity were also evaluated. The extracts of Cinnamomum zeylanicum and Lysimachia christinae Hance were selected as antimicrobial agents for natural food preservatives due to their antimicrobial activity against the tested bacteria and high DPPH radical scavenging activity. Using the natural antimicrobial mixture (0.1% chitosan + 100ppm grapefruit seed extract + 0.2% adipic acid), two kinds of solution with 0.1% Cinnamomum zeylanicum (P1) or 0.1% Lysimachia christinae Hance (P2) were made to investigate the possibility as food preservatives. Viable cell of S, aureus reduced about 5~6 log cycle after treatment for 5 min with P1, or P2 but E. coli did not detected after the same treatment, The above mentioned pathogens did not detected after treatment for 5 min with P1 or P2.