Objectives: This study examines the relationship between the implementation of pit and fissure sealant in children, and the prevention level of teeth based on household income by a cross-sectional study. Methods: The study population consisted of a representative community sample of 1,701 subjects, ranging from 6-18 years in children and over 27 years in mothers, from the 4th Korea National Health and Nutrition Examination Surveys in 2009. Mother`s indicators included age, occupation, education, household income, smoking, brushing frequency, oral health screening, self-related oral health, and mother`s concern. We used a multiple logistic regression to identify and quantify the risk factors for the pit and fissure sealant. Results: In multiple analyses, age of children, household income level, and oral health screening were significant factors for the implementation of pit and fissure sealant for the children. The multiple-adjusted odds ratio (95% confidence interval) for the implementation of pit and fissure sealant, comparing the lowest to 101-200, 201-300, 301-400, 401-500 and 501 ten thousand Korean Won or more were 2.2 (1.15-4.10), 2.5 (1.38-4.61), 2.0 (1.04-3.90), 2.8 (1.46-5.53), and 3.6 (1.82-6.94), respectively. Conclusions: It was concluded that the implementations of the pit and fissure sealant were positively related with the household income level and mother`s oral health screening.