Objectives: It has been proven that doing exercise regularly is effective in lowering the risk of cardiovascular disease. The purpose of this study is to evaluate the relationship between physical activity and the cardiovascular risk factors. Methods: The study samples were 404 men and women who went to Yonsei Fitness Center from May of 2009 to April of 2010. We tried to assess the relationship between physical activity and cardiovascular risk factors. We used paired t-test and multiple regression, Results: The result showed that the significant relationship between the mean value of body weight and the frequency of gym visit in men (mean of 75.9±8.3 in 2009 vs. 74.1±8.6 in 2010 (p=0.0067). Men who used 90 times in gym showed a significantly lower risk (GOT: p=-7.76978, p=0.0 629 GPT: 13=-12.45055, p=0.0003 GGT: p=-9.94185, p=0.0 194) of liver function vs. those who used 1-29 times. Women who used 60 times in gym showed a significantly lower risk (total cholesterol: 13=-28.265l3, p=0.0066; LDL cholesterol: 13=-23.69l64, p=0.0071) of lipid profile vs. those who used 1-29 times. Conclusions: Increased physical activity influences in lowering body weight, lipid profile and improving liver function. We need to conduct follow up research about physical activity program in the work place.