In this study, antibacterial activity and cytotoxicity of the extracts from pericarp and seed of Vitis coignetiea, which were extracted with 0.1% HCl-60% ethanol, were analyzed. The antibacterial activity of the extracts was determined by paper disc diffusion method against food spoilage and food-borne pathogens. The pericarp extract showed high antibacterial activity against Bacillus cereus, Escherichia coli O157:H7, and Pseudomonas aeruginosa, and the seed extract represented the high antibacterial activity against B. cereus, E. coli O157:H7, and Staphylococcus aureus. The cytotoxicity of the Vitis coignetiea extract against human cancer cells was determined using the MTT assay and SRB assay. The pericarp extract represented strong growth-inhibition activity against G361 and Hep3B cells and the seed extract greatly inhibited the growth of HeLa and G361 cells in the MTT assay. In addition, the pericarp extract displayed a high inhibition activity against the growth of AGS cells and the seed extract greatly inhibited the growth of HeLa, Hep3B, and MCF7 cells in the SRB assay. Especially, the cytotoxicities of the seed extract against HeLa were significantly higher than those of the extract against other cancer cells at all test concentrations. This study demonstrates that the extract from pericarp and seed of Vitis coignetiea possess high antibacterial activity and cytotoxicity.