The aim of the present study was to investigate effect of running speed conditions on the kinematic pattern of the metatarsus, mid-foot, calcaneus. Twenty-two healthy young adults were made to run on treadmill at three different running speeds (normal speed, 9.2; slow speed, 7.4; fast speed, 11.1km/hr.) and the trajectories of the 10 reflective markers for each subject were recorded by an eight-camera motion capture system at 200 Hz. Three-dimensional angles for the foot segment in the support phase during running were calculated according to Euler`s technique. Results showed that running speed did not affect the peak of the dorsi/plantar flexion, inversion/eversion, and adduction/abduction or their range of motion for each foot segment. However, when the running speed was fast, significant differences were found in the peak of the plantar flexion, eversion, and adduction and ROM (range of motion) of the dorsi/plantar flexion, inversion/eversion, and adduction/abduction between the foot segments, metatarsus, mid-foot, and calcaneus. It was proposed that the foot segment should be analyzed from a multi-segment system point of view on the basis of anatomical reference during locomotion.