This study was performed to investigate the possible liver protective effect of Holotrichia diomphalia Bates water extract an carbon tetrachloride induced hepatotoxicity in male rats. The water extract of H. diomphalia Bates was orally administrated everyday and carbon tetrachloride, a hepatotoxic agent, was intraperitoneal injected every three day. The effects of H. diomphalia Bates water extract an enzyme activities, bilirubin contents in serum and analyzed of protein patterns by SDS-PAGE were examined. The results were as follows 1. The AST, ALT, LDH and ALP activities in serum were increased by carbon tetrachloride injection, but they were significantly decreased by administrations of H. diomphalia Bates water extract (P$lt;0.01). 2. Bilirubin contents in serum was increased by carbon tetrachloride injection, but it was significantly decreased by administrations of H. diomphalia Bates water extract (P$lt;0.01). 3. Protein band analysis by SDS-PAGE showed main banding between molecular weight 68,000∼43,000 dalton. 4. The Scanning results and number of band by densitometer showed that normal group and 6 day treated group, CCl₄ treated group and 9day treated group were very resemblance, respectively. Therefore, these results suggested that H. diomphalia Bates prevent carbon tetrachloride - induced liver damages.