Osteoporosis, caused by ageing and hormone imbalance due to the menopause, has been known to be able to be cured and protected by prescription of calcium and vitamin D. To develop several organic salts as a raw material for the therapy of osteoporosis, citric acid and sodium gluconate, based on the substitution reaction, reacted with proper inorganic salts such as calcium chloride, magnesium sulfate and zinc sulfate and then the resultants were analyzed by thin layer chromatography. In case of the analysis of Mg, Fe, Zn and Cu(Ⅱ), the mixture of dimethylsulfoxide (DMSO) and HNO3 having the different mixing ratio, were used as the developing reagent and proper color reagent was used according to each salt. Meanwhile, in case of Mg. Ca, Cu(Ⅱ) and Zn, the developing reagent and color reagent were used 5% 2N HCl tertiary-butanol and lithium tetracyanoquinodimethanide [Li(TCNQ)], respectively. In advance, determination of more accurate recipe and clinical trial, based on the quantitative analysis such as high performance liquid chromatography (HPLC), would be needed for developing the agent for the therapy of osteoporosis.