The purpose of this study was to survey pre-service biology teachers` perceptions about biotechnology. The instrument consisted 26 items which were 5-point Lickert scale. Subjects were 107 sophomore and junior students, who were enrolled in universities of Chungcheong, Daegu and Kyeognam provinces. The study revealed that pre-service biology teachers` perceptions were positive toward the use of biotechnology on biological objects such as plants, grains, microbes and animals. However, their perceptions were negative toward the use of biotechnology on humans. The study also revealed that pre-service biology teachers had positive perceptions about the use of biotechnology in the development of beneficial products. Regarding the reliability of biotechnological information acquired from university professors and biologists, they tended to be positive. They perceived that the use of biotechnology should not be affected by religious and ethical problems, even though they perceived that the use of biotechnology should be considered with food safety and genetic factors of microbes. Thus, the results of this study suggested that the development of teaching contents and programs about related to biotechnology be promoted in order to enhance pre-service biology teacher`s performance and teaching contents and programs should be developed for them in biology teacher education.