This study was conducted to characterize the discharge feature of pollutant load from the upper and lower watershed influencing on the water quality of South Han River using the empirical equation and Factor Analysis. The results of regression analysis between flow rate and pollutant load were as follows. In the streams of the upper watershed of South Han river, BOD5 and CODMn were increased as the flow rate was increased. Also, steep increases in SS and TP were observed with positive correlation with the flow rate while change in TN was slightly shown. On the other hand, in the streams of the lower watershed of South Han river, BOD5 was negatively correlated with the flow rate, being decreased with the increase in the flow rate. However, changes in CODMn, TN, SS, and TP showed a similar trend with those observed in the upper watershed. With Factor Analysis of the water quality and various components, it was appeared that the flow rate, SS, and TP were significantly correlated each other and they were indicated as the principal component influencing on water quality in the streams of the upper watershed. In contrast, BOD5, CODMn, and TOC were significantly correlated each other and they were included as the principal pollution component of the streams in the lower watershed. From these results, it was conclusive that the upper watershed of South Han River was mainly affected by non point source pollutants while the lower watershed was influenced by point source pollutants from the developed areas.