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가감신기환(加減腎氣丸) 제형변화가 염증반응 사이토카인과 기억력감퇴에 미치는 영향
The Effects of kagamSinKiHwan(KSKH) Hot water extract & ultra-fine Powder on Proinflammatory cytokine of Microglia & Memory Deficit of Amnesia Mice Model
임현주 ( Hyeon Ju Yim ) , 정인철 ( In Chul Jung )
UCI I410-ECN-0102-2012-510-003000964
* This article is free of use.

Objective : This experiment was designed to investigate the effect of the KSKH hot water extract & ultra-fine powder on microglia and memory deficit model Method : The effects of the KSKH hot water extract on expression of IL-1β, IL-6, TNF-α mRNA and production of IL-1β, IL-6, TNF-α in BV2 microglial cell line treated by lipopolysacchaide(LPS) were investigated. The effects of the KSKH hot water extract & ultra-fine-fine powder on the behavior of the memory deficit mice induced by scopolamine and AChE in serum of the memory deficit mice induced by scopolamine were investigated. Results : 1. The KSKH hot water extract suppressed the expression of IL-1β, IL-6, TNF-α mRNA in BV2 microglial cell line treated by LPS. 2. The KSKH hot water extract suppressed the production of IL-1β, IL-6, TNF-α in 100㎍/ ㎖ concentration of BV2 microglial cell line culture supernatant. 3. The KSKH hot water extract & ultra-fine powder decreased AChE activation significantly in the serum of the memory deficit mice induced by scopolamine. 4. The KSKH hot water extract & ultra-fine powder showed significant effect on memory impairment in the stop-through latency type of Morris water maze test. Conclusions : This experiment shows that the KSKH hot water extract & ultra-fine powder might be effective for the prevention and treatment of amnesia and Alzheimer`s disease. Investigation into the clinical use of the KSKH hot water extract & ultra-fine powder for amnesia and Alzheimer`s disease is suggested for future research.

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