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천왕보심단(天王補心丹) 열수추출물(熱水抽出物), 초미세분말제형(超微細粉末劑形)이 Alzheimer`s Disease 병태(病態) 모델에 미치는 영향(影響)
The Effects of ChenwhangBosindan(CBD) Hot Water Extract & Ultra-fine Powder on the Alzheimer`s Disease Model
최강욱 ( Kang Wook Choi ) , 정인철 ( In Chul Jung )
UCI I410-ECN-0102-2012-510-002990474
* This article is free of use.

Objective : This experiment was designed to investigate the effect of the CBD hot water extract & ultra-fine Powder on Alzheimer`s Disease Model Induced by βA. Method : The effects of the CBD hot water extract on expression of interleukin-1 beta(IL-1β), TNF-α mRNA and production of IL-6, TNF-α in BV2 microglial cell line treated by lipopolysacchaide(LPS). The effects of the CBD hot water extract & ultra-fine powder on (1) the behavior (2) expression of IL-1β, tumor necrosis factor-alpha(TNF-α), (3) the infarction area of the hippocampus in Alzheimer`s diseased mice induced with βA were investigated. Result : The CBD hot water extract suppressed the expression of IL-1β, TNF-α mRNA in BV2 microglia cell line treated with LPS. The CBD hot water extract significantly suppressed the production of IL-1β, TNF-α in BV2 microglial cell line treated with LPS. The CBD hot water extract & ultra-fine powder a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer`s disease induced by βA in the Morris water maze experiment, which measured step-through latency and distance movement-through latency. The CBD hot water extract & ultra-fine powder significantly suppressed the expression of IL-1β and TNF-α protein in the microglial cell of mice with Alzheimer`s disease induced by βA. The CBD hot water extract & ultra-fine powder suppressed the over-expression of AChE activity in the serum of the mice with Alzheimer`s disease induced by βA. The CBD hot water extract & ultra-fine powder reduced infarction area of hippocampus, in the mice with Alzheimer`s disease induced by βA. Conclusions : These results suggest that the CBD hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer`s disease. Investigation into the clinical use of the CBD hot water extract & ultra-fine powder for Alzheimer`s disease is suggested for future research.

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