The spray development, mixture preparation and combustion characteristics in a four-stroke direct injection spark ignition (DISI) engine with a multi-hole injector of symmetrical and asymmetrical nozzle-hole arrangements, including the nozzle hole-hole angles of 54o, 57o and 60o, have been investigated by the computational fluid dynamics (CFD) codes. Meanwhile, to form homogeneous air-fuel mixture, the simulation was completed at the injection starting of 30CAD ATDC intake stroke. The simulated results show that, when the fuels are injected during the early intake stroke, with the included angle decreasing, it seems to have the worse impact on the mixture preparation and combustion, due probably to the spray-spray interaction and various spray impingements leading to the worse fuel evaporation and atomization. Hence, it could be concluded that the nozzle holes arrangement is a key factor for the multi-hole injector. Besides, to verify the spray model validity the spray experiment with a 57o nozzle included angle, multi-hole injector was carried out in a quiescent optical chamber, using the high-speed shadow photography. The results show that the calculated values are in agreement with the experimental ones, verifying that the spray calculation model is reasonable.