In 2005, 90 isolates of Colletotrichum gloeosporioides causing persimmons tree anthracnose were obtained from infected twigs and fruits of persimmon trees. Their responses to nine fungicides, consisting of two benzimidazoles (thiophanate-methyl, carbendazim), three ergosterol-biosynthesis inhibitors (difenoconazole, myclobutanil, tebuconazole), and four protective fungicides (propineb, mancozeb, chlothalonil, and dithianon), were investigated with relative mycelial growth index to untreated control on PDA treated with field application rate of each fungicide. At response to carbendazim (415 μg/ml) and thiophanate-methyl (750 μg/ml), 82% and 78% of isolates showed relative mycelial growth index under 0.1 to untreated control, respectively. All of them did not grow on PDA incorporated with myclobutanil (40 μg/ml) and tebuconazole (75 μg/ml). Only one isolate (PER 36) grew on PDA amended with difenoconazole (50 μg/ml), but its relative mycelial growth index to untreated control was very low with a values of 0.03. They were most sensitive to propineb (1,500 μg/ml) among four protective fungicides.