The pathogenesity of 25 isolates of Colletotrichum gloeosporioides from apple, 42 isolates from pepper, 5 isolates from jujube, 8 isolates from persimmon was evaluated to know transmission to strawberry from other infected plants. Followings are the results. Colony morphology and spore size on potato dextrose agar was similar. When each isolate was inoculated on leaf and petiole of strawberry, isolates from persimmon was the most pathogenic. Five isolates, one pathogenic isolate per each host, were evaluated in simulated field condition under natural rainfall for their natural infectivity. All isolates infected strawberry in field condition, so C. gloeosporioides from other hosts are potential inoculum source of strawberry anthracnose.