The commercial antiseptic solution samples containing hydrogen peroxide were analyzed in relation to the hydrogen peroxide content, using the various plant pigments, red rose petals, red wine, purple cabbage, azalea petals with spectronic 20 and home-made LED colorimeter. The results suggest that any anthocyanine-containing plants can be used in hydrogen peroxide determination. The negative sign in the slope of the analytical curves represents the decrease in the absorbance, resistance caused by the discoloring reaction. This study could easily determinate about H2O2 containing commercial hydrogen peroxide water with calibration curve from spectronic 20 and home-made LED colorimeter. It avail student`s determinative understanding about oxidation-reduction chapter.