The research assessed water quality using physico-chemical factors, trophic status index and attached diatom index in 10 reservoirs located in Chungcheongnam-do from October 2007 to June 2008. The physico-chemical water quality assessments revealed that Dangjin-gun (St. 1~5), which displayed a high chemical oxygen demand (V and VI) and chlorophyll-α (Eutrophic-Hypereutrophic), was more polluted than Yesan and Cheongyang-gun. The sample were also hypereutrophic [trophic status index (TSI) 74.6] and β-mesosaprobic [diatom assemblage index to the organic water pollution (DAIpo) 44.47]. Yesan and Cheongyang-gun. Which were mid-level in their pollution status, were eutrophic (TSI 56.9) and α-oligosaprobic (DAIpo 60.11). TSI correlated strongly with the attached diatom index (of DAIpo 0.60-0.62, and trophic diatom index of 0.72~0.74). Hence, the attached diatom index can be used with the trophic status assessment of lakes using TSI, and lake trophic status index (LTSI).