A high prevalence of protected horiculture farmer`s work-related musculo-skeletal disorders (MSDs) have been reported in precedent studies. One of the tasks required ergonomic intervention to reduce the musculo-skeletal risks is the task of harvesting. The purpose of this study is to evaluate quantitatively the spinal load of worker harvesting with squatting posture to predict and prevent musculo-skeletal risks. Spinal load in Squatting posture with asymmetric trunk motion were analyzed. Before evaluating spinal load on harvesting worker by bio-mechanical approach, it is needed to validate human model. In this study, ADAMS LifeMOD human model shows satisfactory results, comparing with already validated model`s results or measured results. While worker reached arms (20%, 40%, 70% arm reach) with various asymmetric trunk motion (0, 45, 90 degree), their spinal loads (extension, twisting and lateral bending moment) were evaluated. In case of extensor moment at lumbo-sacral joint, the more the arm reach got increased, the moment increased. however, in case of twisting moment and lateral bending moment, the more both arm reach and asymmetric trunk motion got increased, the moment increased significantly. The findings of this study suggest that it need to be determine the spinal load, especially twisting, lateral bending moment in evaluating musculo-skeletal workload in squatting posture.