Objectives: This study purposed to research the inhibitory effect of Berberidis Ramulus on the liver cancer. Methods: A total extract of Berberidis Ramulus decoction were prepared. Through the measurement of the cell proliferation, apoptosis, morphology and cytokine level from the extracts, the influence on HepG2 cell were compared. Results: Berberidis Ramulus extract significantly inhibited the proliferation, increased the apoptosis, decreased the TGF-b gene expression and the K-RAS gene expression, significantly increased the level of TNF-a secretion and increased the rates of IFN-y secretory cells. Conclusion: It is suggested that Berberidis Ramulus extract turned out to have anti-cancer effects on HepG2 cell.