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IgY 투여에 따른 포유자돈의 설사에 대한 예방효과 -1. 혈청학적 결과, 형광항체검사 및 RT-PCR 검사
Protective effects of IgY against diarrhea in suckling piglets -1. Serological result, FA test and RT-PCR
김문 ( Wen Jin ) , 윤병일 ( Byung IL Yoon ) , 한정희 ( Jeong Hee Han )
UCI I410-ECN-0102-2012-490-001588508

The purpose of this study was to investigate the protective effects against porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) in suckling piglets by oral administration of IgY. Twenty piglets were divided into two groups with the same number: group I(treated with IgY) and group II(not treated). Group I was administerd orally with IgY for three days from one-day-old and experimentally chal-lenged with PEDV and TGEV at four-day-old. The other was administered with saline solu-tion and challenged with PEDV and TGEV at four-day-old. Serum antibody titers against PEDV and TGEV were examined by enzyme-linked immunosorbent assay(ELISA) and the detection of PEDV or TGEV antigen from feces and small intestines was performed by reverse transcription-polymerase chain reaction(RT-PCR) and indirect immunofluores-cence(IFA). The antibody titers of the group I was higher than that of the other, and lasted at the end of experiment. In the detection tests of both virus from feces and small intes-tine, the rate of the group I was lower. Based on these results, oral administration of IgY may be effective to prevent the diarrhea caused by PEDV and TGEV.

[자료제공 : 네이버학술정보]
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