Bovine leukosis is one of the important diseases in Korea because of economic losses, and this study was conducted to provide basic information for the control of the disease. A total of 2,104 sera were obtained from 491 farms and examined by commercial kit. In the seroprevalence of farms and heads, 71 farms out of 491 were seropositive (14.5%) and 119 heads were positive (5.7%) out of 2,104. In the areal distribution of seropositiveness, Soseong-myeon showed the highest rate (39.5%, 15/38 farms)and Yeongwon-myeon was the lowest (2.6%, 1/38 farms). By age, positive rate in the below of 1 year old cattle was much less than that in the over of 1 year old, namely, the former was 1.9% (3/161 farms) and the latter 20.6% (68/330 farms). According to the farm size, there was no significant difference among the size. However, the highest rate was shown in the farms having 50-99 heads, 23.2% (13/56 farms).