This survey was carried out to investigate the seroprevalence of antibodies to Neospora caninum in Korean indigenous cattle that was representative livestock raised in Jeongeup province Jeonbuk Korea. A total of 1,162 sera were tested for N caninum antibodies using ELISA(Herdcheck anti-Neospora, IDEXX Laboratories Inc., Westbrook, Maine USA). 15(1.3%) sera were positive by ELISA. A total of 290 farms were tested, 11(3.8%) farms were positive. Among the seroprevalence of cattle according to the areas, 8 of 44 counties were infected N caninum. Sero-positive 11 farms of N caninum antibodies using ELISA test and 62 of 301 sera (20.6%) were infected to N caninum. We found that cattle be raised in Jeongeup province was slightly infected to N caninum. It seems to be infected of N caninum an early stage, but the positive rates was variable that it was 6%~62%. It was supposed to the highest positive rate of 3 farms had some factors to be infected. The factors were supposed to keep dogs yearly, located the base of hills, and almost feed with imported dried grass. Farmers suppose to the cause of abortion was not the neosporosis but also broke out an accident or was infected Brucella spp. The results of Brucella Rose Bengal Test(RBT) were all negative.