Porcine E. coli infection is a disease caused by Enterotoxin produced by Enterotoxigenic Escherichia coli (ETEC). Enteric colibacillosis has become an economically important disease in pigs as a result of increasing intensification of farrowing management. The present study undertaken to obtain the antibiotic sensitivity and distribution of serogroups and pili producibility test of ETEC from E. coli isolates in Chonnam. The results obtained were as follows. 1. A total of 71 isolates identified as E. coli employing IMViC system from rectal specimens of 54 piglets with diarrhea. 2. In antibiotic sensitivity test, isolates showed high sensitivity to AN, CM, Fox, GM, but resistance to EM, NA TC. 3. The distribution of 25 isolates of serogroups were 0141:K85(11.3%), 08:K87(8.5%), 064:K-(5.6%), 0138:K81(4.2%), 0139:K82(2.8%), 0157:K88ac(1.4%) and 0149:K91(1.4%). 4. MRHA of guinea pig erythrocytes was detected in 8 out of 25OK serotypes and 9 out of 46 unidentified serotypes. MRHA titers of serotypes showed from 64 to 128 in 0141:K85, 2 in 0138:K81 and no titers in 0139:K82. 5. The production of heat labile enterotoxin of ETEC was detect 39 out of 52 isolates showed β-hemolysin, 7 out of 52 isolates showed γ-hemolysin and 6 out of 52 isolates showed γ-hemolysin by GM1 ganglioside ELISA. The distribution of LT toxin were in 12 isolate showed β-hemolysin, 2 isolates showed α-hemolysin and 3 isolates showed γ-hemolysin in 25OK serotypes.