The purposes of this study were to describe the effects of perception of organizational culture type and job satisfaction on university hospital nurses` innovation behavior. A survey was conducted with 418 respondents working in a university hospital in Cheonan City. Organizational culture types (developmental, group, rational, hierarchical), job satisfaction and individual characteristics were surveyed. The relationships between each variable and the innovation behavior were analyzed by univariate analysis and the independent effects of these variables were examined with multiple regression. The mean score of general employees innovation behavior was 3.33 (p=.58) and that of managers was 3.53(p=.64). For general employees, there were statistically significant differences in innovation behavior according to education level. For managers, there were statistically significant differences in innovation behavior for education level, marital status and duration of work. On multiple regression analysis, the factors affecting innovation behavior of general employees were developmental culture(β=.297, p<.01), group culture(β=.184, p<.01) and job satisfaction(β=.148, p<.05). And the factors affecting innovation behavior of managers were developmental culture(β=.181, β p<.01), rational culture(β=.171, p<.01) and group culture(β=.408, p<.01), In conclusion, the results of this study show that organizational culture and job satisfaction influence hospital nurses` innovation behavior