The purpose of this study was to analyze muscle activities on functional insole with diet effect. Originally, ten healthy female subjects with an average age of 23.2 year(S.D=1.1), weight of 49.7 kg(S.D=4.9), height of 163.2 cm(S.D=3.5) and a shoe size of 237.5 cm(S.D=4.9) were participated in this experiment. Ten healthy females walked on a treadmill(speed=about 4.2 km/h) wearing two different insole types. Muscle activities data was collected using the EMG operating system. The surface EMG signal for tibialis anterior(TA), gastrocnemius(GA), vatus lateralis(VL) and biceps femoris(BF) were acquired at the RMS(10 Hz, 350 Hz) using Noraxon Telemyo DTS system(Noraxon inc, USA). This study processed the data using the Windows SPSS ver.17.0 to get an independent t-test, with the setting, p<.05. Analysis of muscle activity were measured and calculated during walking. The results are as follow: Functional insole wearing were increased muscle activities significantly from Tibialis anterior(TA) during total gait cycle. Normal distribution was demonstrated in total step of stances period. One foot standing position showed decreased muscle activity. Two foot standing position was demonstrated with gastrocnemius and biceps femoris. As a result of the analysis, Functional insole will inerease the diet effect in the use of four muscle groups.