Objectives : The purpose of this study is to observe the anti-oxidative effects of electroacupuncture at GB34(Yangreungcheon) in rats. Methods: The authors performed several experimental items including measurements of body weight, relative Liver weight, Levels of albumin, total bilirubin, LDL-cholesterol, LDH, GOT and GPT in blood serum, Levels of SOD, glutathione, catalase, NO and MDA in Liver, and histological analysis of Liver. The conclusions are as follows. Results: 1. In the GB34-EA group, Liver index was Lower than those of the control group and the holder group. 2. In the GB34-EA group, the Levels of albumin and total bilirubin in serum were increased significantly compared to those of the control and the holder group. LDL-cholesterol Level was decreased significantly compared to those of the control group and the holder group. 3. In the GB34-EA group, Liver SOD, glutathione, catalase activity were increased, and NO concentration in Liver was decreased significantly compared to those of control and the holder group. 4. In the GB34-EA group, the density of Liver tissue was more similarly maintained to that of the normal group compared to those of the control group, holder group and sham-EA group. Conclusions: These results suggest that electroacupuncture at GB34 may have an antioxidant effect in rat.