Objectives: The objective of the current study is to optimize an acupoint electrical property measurement system by using the procedures and strengths of the Taguchi method. Methods: Taguchi method was applied in the optimization of the acupoint electrical property measurement system, as in the following steps: (1) determine evaluation index; (2) execute the 1st gage R&R; (3) identify controllable and uncontrollable factors; (4) design Taguchi crossed array Layout; (5) execute experiments according to trial conditions; (6) analyze testing results; (7) determine optimum run; (8) execute the 2nd gage R&R; (9) confirm optimum run. Results: The results showed that electrodes with Larger diameter and efferent electric current, with music played, can produce the Larger different electrical conductivity between the acupoints and the non-acupoints under various conditions. Taguchi process optimization improved repeatability and reproducibility of the acupoint electrical property measurement system. Conclusions: In the optimized condition, repeatability and reproducibility of the acupoint electrical property measurement system was acceptable.