Objectives: Ulcerative colitis or Crohn`s disease has been recognized as Ha-ri (下痢) or Jang-Byok (腸벽) in Korean oriental medicine. A purpose of the present study is to investigate the anti-inflammatory effect of moxi-tar herbal acupuncture at LI4 (HapGok) on 2,4,6-trinitrobenzene sulphonic acid (TNBS) induced colitis in rats and further elucidate the possibility of herbal acupuncture on ulcerative colitis which is chronic inflammatory disease of the gastrointestinal tract. Methods: Sprague-Dawley rats, weighing 170~190 g, were subjected to intrarectal injection of either saline (300 ㎕, 500 ㎕) for a control or 2,4,6-trinitrobenzene sulphonic acid (TNBS) (300 ㎕, 500 ㎕) for a colitis. Moxi-tar herbal acupuncture at bilateral LI4 was used as the treatment. A volume 0.4 ㎖of moxi-tar (20 ㎎/㎖) were subcutaneously injected to the LI4 just after the secondary injection time of TNBS in rats. To study the effects of Moxi-tar acupuncture in LI4, body weight, RBC count, WBC count, total protein, Paw edema rate, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels were observed. Results: Moxi-tar acupuncture in LI4 on TNBS-induced colitis inhibited the body weight lose rate but not effect RBC, WBC count. In addition, it inhibited the reduction of total protein concentration, paw edema, rate of protein leakage into CMC-pouch fluid, IgG levels and IgM levels. Conclusions: It is suggested that moxi-tar herbal acupuncture at LI4 helps to recover TNBS-induced colitis and plays an important role for an treatment of the irritable bowel syndrome (IBS).