Objective: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased NO and iNOS generation in C6-glioma and RAW 264.7 cells in our Lab. The purpose of this research was to investigate the protect reaction on cell injury induced by the H2O2 in C6-glioma cells. Methods: C6-glioma cells were cultured in RPMI 1640 with FBS 10% in CO2 incubator. To study the protective effects of moxi-tar, we observed cell viability, DPPH activity, SOD activity, catalase activity and cell morphology after injury with H202. Results: Moxi-tar increased cell viability about twice as much as that of being injury by H2O2 (moxi-tar 40 μg/ml, H2O2 500 μM). And the results of free radical scavenger activity (80 μg/ml: 78.91±4.4%), SOD activity and catalase activity (80 μg/ml: 21.6 unit/mg protein) were increased by moxi-tar as dose-dependent manner. Conclusion: we concluded that the effects of moxibustion which is played important role in Oriental medicine, might be free radical scavenger effects induced by moxi-tar.