발암물질을 무독화시키는 QR 생성 유도를 살펴보기 위하여 川芎 약침액 및 열수추출액을 생쥐의 간암세포인 Hepa1c1c7에 처리하여 측정한 결과, 황기 약침액의 농도를 증가시킬수록 많은 QR 생성율을 보였으며, GST 생성 또한 증가하였다.

Induction of phase Ⅱ, enzymes such as quinone reductase (QR) or glutathione S-transferase (GST) is considered a major mechanism of protection against initiation of carcingenesis. The present study was performed to evaluate the chemopreventive activity of Cnidium officinale Makino aqua-acupuncture solution (COMAS) and Cnidium officinale Makino water-extracted solution (COMWS) in vitro. COMAS and COMWS are potent inducer of quinone reductase and glutathioe S-transferase activities in murine hepatoma Hepa1c1c7 cells. However, GSH activity was not increased with COMAS and COMWS. These results suggest that COMAS and COMWS may act as blocking agents against carcinogenesis by induction of phase Ⅱ enzymes.