Objectives: Miso facial rejuvenation acupuncture is a Korean medical treatment technique for face lifting. The authors aimed to evaluate the effects of Miso facial rejuvenation acupuncture on facial skin furrows. Methods: 11 patients who made first visits to Hyo-Jun Korean Medical Clinic from October 1, 2009 to October 25, 2009 were each given one-time treatment of Miso facial rejuvenation acupuncture treatment. A portable fluorescent dermal diagnostic equipment, ECOSKIN, was used to measure extraocular skin furrows and perioral skin furrows width and depth before and after treatment. Results: Mean width and depth of both the extraocular and perioral skin furrows decreased after one-time Miso facial rejuvenation acupuncture treatment. Extraocular skin furrow width, depth, and perioral skin furrow width showed statistically significant decreases(p=0.003, 0.017, 0.041 respectively). Conclusions: Mean depth and width of both the extraocular and perioral skin furrows decreased after receiving Miso facial rejuvenation acupuncture treatment. Miso facial rejuvenation acupuncture may be an effective treatment for improving wrinkles in clinic.