This study was carried out to confirm whether enzyme activities and gene expression are well correlated to the degree of hair growth in a shaving animal model of C57BL/6 mice. Six weeks old male mice were divided into four groups including normal group(saline), vehicle control group(jojoba oil), positive control group(3% minoxidil) and experimental group(3% mixture oil of peppermint and rosemary in jojoba oil). ALP and γ-GT activities in anagen phase were significantly increased in PC and E groups compared to N or VC group (p<0.05). IGF-1 expression in anagen phase was also significantly increased in PC and E groups compared to N or VC group (p<0.05). The parts and degree of SCF antigen expression were more extensive and stronger in E and PC groups compared to N or VC group. These results indicated that ALP and γ-GT activities and IGF-1 expression in the skin of a shaving animal model of C57BL/6 mice were well correlated to the degree of hair growth and good biomarkers in anagen phase.