Nurses evaluate the physical, emotional, and spiritual well-being of a person when conducting an assessment and forming a plan of care. Nursing is a holistic approach to health and well-being. Implication for Nursing Practice: nurses must assess and support intrinsic religiosity and promote spiritual well-being in peoples coping with severe diseaser. The purpose of this study was to investigate spiritual well-being, hope and self-esteem of nursing students, and to identify spiritual well-being, hope and self-esteem the differences between nursing students of christian university and nursing students of non-christian university. The measurment tools for spiritual well-being, hope anf self-esteem were a self-report questionnaire. The collected data was prepared for computer analysis and analyzed using appropriate statistical methods. General characteristics, spiritual well-being, hope and self-esteem are analyzed by descriptive statistical methods. For hypothesis testing t-test, Pearson correlation are used. The result of this study can be summarized as follows; 1. The mean score for spiritual well-being in the nursing students was 79.52 of a possible range of 20-120. And the mean score for hope was 58.18 of a possible range of 29-116, the mean score for self-esteem was 112.29 of a possible range of 30-150. 2. "Nursing students of christian university will demonstrate higher spiritual well-being than the nursing students of nonchristian university" was rejected(t=1.01, p=.96). 3. "Nursing students of christian university will demonstrate higher hope than the nursing students of nonchristian university" was rejected(t=1.71, p=.05). 4. "Nursing students of christian university will demonstrate higher self-esteem than the nursing students of nonchristian" was rejected(t=1.53, p=.12). 5. "The higher spiritual well-being, the higher hope" was rejected(r=-.664, p=.000). 6. "The higher spiritual well-being score, the higher self-esteem" was supported(r=.487, p=.000).